First, we iterate through the incoming state parameter rather than the local #data. That’s because if the incoming state is missing a key that #data has, we know that we don’t need to touch that key.8
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This reaction would contain only low concentrations of the dNTPs and relied upon brief incubation times, so that DNA synthesis would stall at random positions along the template and yield a population of DNA fragments with varying lengths. These unfinished DNA fragments were then purified and used as templates in four “minus” and four “plus” reactions.，更多细节参见safew官方版本下载